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KMID : 0604519960220020201
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Journal of the Society of Cosmetic Scientists of Korea
1996 Volume.22 No. 2 p.201 ~ p.210
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Three Predictive Tests Using Mice for the Identification of Contact Sensitizer
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Shin, Jung-Hyun
Lee, Min-Geol/Jung, Sang-Wook/Park, Mun-Eok/Lee, Cheon-koo/Kang, Seh-Hoon
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Abstract
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Predictive tests for the identification of contact sensitizing devel oped. We measured the sensitization potential with three vitro and the in vivo Local Lymph Node Assay(LLNA), ELISA to detect interferongamma(IFN-γ) from supernatant and flow cytometry to detect change of cell surface proteins, using draining lymph nodes of mice.
BALB/c mice were exposed to various chemicals or vehicles on the ears daily for 3 consecutive days in all experiments. With some exceptions of propyl paraben, neomycin sulfate, the in vivo LLNA was able to detect the sensitizing capacity of test chemicals and was more sensitive than the in vitro LLNA for chemicals used in the present study. In another experiment, contact sensitivity was assessed by the ELISA to detect IFN-γ from the supernatants of the cultured LNCs after sensitization with chemicals. There was a good correlation between the LLNA and the IFN-γ production for test chemicals. We also examined the change of cell surface proteins on LNCs after sensitization by flow cytometry for some cell adhesion molecules(ICAM-1, E-cadherine, B7 molecule), T cell markers(CD3, CD4, CD8, Tαβ, Tγδ) and B cell markers(LR1, CD45R, I-A^(d) ). The number of ICAM-1 positive cells and B cells in LNCs were increased after sensitization with DNCB, TNCB, isoeugenol and 25%, 50% cinnamic aldehyde compared with that of vehicle as a control.
In conclusion, the in vivo LLNA could provide more sensitive screening test for moderate to strong sensitizers and some weak sensitizers including cosmetic raw materials than the in vitro LLNA. The production of IFN-γ by allergen-activated LNCs might be a valuable indicators without radioisotopes for the identification of contact allergens. Detection of allergens by testing the increase of ICAM-1 positive cells and B cells in LNCs by flow cytometry might be used as a test method to detect allergens.
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